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Bone Healing Research

Human Studies


Abe, T. (1989).
LLLT using a Diode Laser in Successful Treatment of a Herniated Lumbar/Sacral Disc, with Magnetic Resonance Imaging (MRI) Assessment: A Case Report. Laser Therapy. John Wiley & Sons.


Sousa G R, Ribeiro M S, Groth E B. (2002). Bone repair of the periapical lesions treated or not with low intensity laser (wavelength=904 nm). Laser Surg Med. Abstract Issue 2002. abstract 303.


Animal Studies


Ozawa Y; Shimizu N; Kariya G; Abiko Y (1998).
Low-Energy Laser Irradiation Stimulates Bone Nodule Formation At Early Stages Of Cell Culture In Rat Calvarial Cells. Bone 22 (4): 347-354. Abstract: To determine the target cells responsible for the action of laser irradiation and roles of irradiation on these cells during bone formation, we investigated the effects of low-energy laser irradiation at various cell culture stages on cellular proliferation, bone nodule formation, alkaline phosphatase activity, and osteocalcin gene expression, employing rat calvarial cells. Osteoblast-like cells isolated from fetal rat calvariae were irradiated once with a low-energy Ga-Al-As laser (830 nm, 500 mW) at various cell culture stages (days 1-16). Laser irradiation at early stages of culture significantly stimulated cellular proliferation, ALP activity, and osteocalcin gene expression thereafter. Furthermore, laser irradiation at earlier stages of culture significantly stimulated a greater number (1.7-fold) and larger area (3.4-fold) of bone nodules that had developed in the culture dish on day 21. However, these effects could not be found by irradiation at a later date. These results suggest that laser irradiation may play two principal roles in stimulating bone formation. One is stimulation of cellular proliferation, especially proliferation of nodule-forming cells of osteoblast lineage, and the other is stimulation of cellular differentiation, especially to committed precursors, resulting in an increase in the number of more differentiated osteoblastic cells and an increase in bone formation. Both bone-formation-stimulating roles may be exhibited by laser irradiation to immature cells only.


Silva Junior A, Pinheiro A, Oliveira M et al. (2002). Computerized Morphometric Assessment Of The Effect Of Low-Level Laser Therapy On Bone Repair: An Experimental Animal Study. Journal of Clinical Laser Medicine & Surgery, 20 (2): 83-87.


Trelles, M.A. & Mayayo, E. (1987). Bone Fracture Consolidates Faster With Low Power Laser. Lasers in Surgery and Medicine 7 (1) 36-45.


Glinkowski W, Rowinski J (1995). Effect Of Low Incident Levels Of Infrared Laser Energy On The Healing Of Experimental Bone Fractures. Laser Therapy, 7: 67-70.


Chen, J., & Zhou, Y. (1989). Effect of Low Level Carbon Dioxide Laser Radiation on Biochemical Metabolism of Rabbit Mandibular Bone Callus. Laser Therapy. John Wiley & Sons.


David R, Nissan M, Cohen I, Soudry M.(1996). Effect of low-power He- Ne laser on fracture healing in rats. Lasers Surg Med, 19: 458-464.


In Vitro Studies



Bassleer, C., Dachy,M., Reginster, J.Y., Gysen, P., Bassleer, R., Franchimont, P. (1985).
Human Articular Chondrocytes Cultivated in Three Dimensions: Effects of I.R. Laser Irradiation. International Congress on Laser in Medicine and Surgery, Bologna: 381-385.


Laser Research